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Cover

Course Prefix
BIOT
Course Number
70
School Identifier
Course Suffix
L
70L
Open Entry
No
Schools
ILT - Life Sciences and Technologies
Department
IHS - Health Sciences
Subject
BIOTCH - Biotechnology
Program
BIOTCH - Biotechnology
Course Title
Introductory Biotechnology Laboratory
Short Title
Intro BIOT Lab
Course TOP Codes should be aligned with the program. Career Education must use an occupational TOP Code. Please check the TOP Code manual or consult with the Curriculum Specialist for assistance.
CB03 TOP Code
043000 - Biotechnology and Biomedical Technology
CB04 Course Credit Status
D - Credit - Degree Applicable
California Classification Code (CB11)
Y - Credit Course
Effective Implementation date of new/revised course
Fall 2016
Catalog Course Description
This laboratory course addresses basic skills and techniques common to the biotechnology industry. Topics include measurement of activity and quantity of proteins, growth and manipulation of bacteria, genetic engineering and antibody methods. This course is intended for students majoring in applied biotechnology and is the recommended course to accompany BIOT 70.
Class Schedule Course Description
Introduction to common techniques and basic skills common to the biotechnology industry.
Justification of Need
Part of biotechnology certificate program.
Material Fee
0.00
Is this a cross listed course? No

Units/Hours

CB04: Credit Status
D - Credit - Degree Applicable
CB10 Course COOP Work Exp-ED
N - Is not part of a cooperative work experience education program.
This course is variable No
This course is approved for lab workload (1.0) No
If no hours per category enter zero.
Weekly Faculty Contact Hours Lecture Min
0.00
Weekly Faculty Contact Hours Lecture Max
Weekly Faculty Contact Hours Lab Min
3.00
Weekly Faculty Contact Hours Lab Max
Weekly Faculty Contact Hours Learn Ctr Min
0.00
Weekly Faculty Contact Hours Learn Ctr Max
Weekly Faculty Contact Hours Total Min
3.00
Weekly Faculty Contact Hours Total Max
CB07 Min Units
1.00
CB06 Max Units
1.00
Total Min Unit Calculation
Lecture Lab Learn Ctr Total
Weekly Faculty Contact Hours0.003.000.003.00
Total Contact Hours0.0049.800.0049.80
Lecture Hour Equivalent0.002.500.002.50
Full Time Equivalent Faculty0.0016.670.0016.67
Units0.001.000.001.00
Outside of Class Hours0.00
Total Student Learning Hours49.80
Total Max Unit Calculation
Lecture Lab Learn Ctr Total
Weekly Faculty Contact Hours0.000.000.000.00
Total Contact Hours0.000.000.000.00
Lecture Hour Equivalent0.000.000.000.00
Full Time Equivalent Faculty0.000.000.000.00
Units0.000.000.001.00
Outside of Class Hours0.00
Total Student Learning Hours0.00
Maximum Enrollment 28
Anticipated Enrollment 26
Maximum WFCH
84.000
Average WFCH
78.000
Open Entry
No
Grading Method
Letter Grade or Pass/No Pass

Repeatability

Only select YES if non-credit or a qualified KNES Course
Repeatable
No
The Functionally Identical or Fractional Courses are editable by admin only.
Functionally Identical or Fractional Courses
Repeatability Group Information
If this course is part of a Repeatability Group, click the checkbox below.
This course is part of a Repeatability Group No
Repeatability Group

Distance Education

Each mode of instruction for an existing course intended for delivery by Distance Education (DE) shall be separately reviewed and approved by the Curriculum Committee prior to being offered. DE mode of instruction includes any regularly scheduled work that replaces otherwise scheduled class (“face”) time. [per Education Code 55378]
This course is/or proposed for DE

General Education/Transfer

Degree Transfer Applicability
Proposed Transfer Types
Not Transferable, AA-AS Degree Only
Explanation
UC Transferable Course
Y - UC Credit
General Education
UC Approval Date 08/19/2016
CSU Approval Date 08/19/2016
CSU GE Approval Date 08/01/2016
IGETC Approval Date 08/01/2016
Local GE Approval Date
Comparable SC/IVC Courses
Comparable Transfer Courses
Course Number
Articulation College System
4-yr Institution
SC/IVC Code Yes
B1 - Breadth: Natural Sciences
CSU GE Yes
B3 - Laboratory Activity
CSU AI Areas No
IGETC Yes
5C - Laboratory Activity
Cal-GETC No
CID

Requisites

Course Requisites
Prerequisite
Requisite Comment
Prior completion of or concurrent enrollment in BIOT 70
Subject
RequisiteCourse
Parenthesis
Condition
Parenthesis
Conditions of Enrollment Catalog View
 

Requisite Validation

Legacy Requisite Validation

Learning Objectives



Bloom's Taxonomy

Students participating in this class will:
Explain the importance of Good laboratory Practices and record keeping.
Distinguish between accuracy and precision.
Employ a lab protocol and explain deviations from the protocol.
Prepare and analyze graphs.
Demonstrate accurate pipetting skills
Demonstrate use of aseptic techniques in microbiological applications.
Perform a bacterial transformation.
Measure the quantity of isolated protein and nucleic using various lab instruments.
List the basic steps of the Enzyme Linked ImmunoSorbent Assay (ELISA) protocol and describe its use in medical diagnosis of disease or conditions (e.g. AIDS and pregnancy)
Identify microscope parts.
Demonstrate proper use and care of the microscope.

Content

If no fields are showing on this tab, the Lecture and Lab fields on the Units/Hours tab are 0
Course Lecture Content (Use outline format)
Course Lab/Activity Content
  1. Biotechnology overview
    1. Biotechnology careers
    2. Good manufacturing practices
    3. Scientific Method
    4. Distinguish between Quantitative and Qualitative observations
  2. Basic laboratory techniques and metrology
    1. Good Laboratory Practices
    2. Lab safety guidelines
    3. pH meter
    4. Record keeping
    5. Accuracy verses precision
    6. Pipetting skills
    7. Spectrophotometer
    8. Metric system
    9. Graphs
    10. Microscope use and maintenance
  3. Protein technique
    1. Protein assays
    2. Introduction to enzymes
    3. Protein electrophoresis
  4. DNA techniques
    1. Basic bacteriology
    2. DNA electrophoresis
    3. Bacterial transformation
    4. Isolation of plasmid DNA/restriction digest
  5. Antibody methods
    1. ELISA assay
    2. Home pregnancy test

Assignments

Typical Outside-of-Class Assignments
Reading Assignments
Laboratory instructions from lab manual, Equipment instructions, Web articles, standard operating procedures.
Writing Assignments
Responses to worksheets questions based on interpretations of the results. Daily recordings in a laboratory notebook explaining lab protocols and analyzing results obtained. Written standard operating procedure document of an assigned method.
Oral Assignments
Data compilation, graph preparation and interpretation.
Other Assignments
Present individual team's findings to class.

Method of Evaluation

Legacy Methods of Evaluation
  1. Instructor evaluation of organization of student's experimental results in the lab notebook.
  2. Instructor evaluation of student generated standard operating procedure that demonstrates the student's ability to accurately explain the use of equipment or a procedure for an experimental process.
  3. Instructor assessment of the student's lab notebook that demonstrates the ability to interpret  experimental results, follow a protocol and explain deviations from the protocol. 
  4. Instructor evaluation of the student's ability to  compile data, produce graphs and interpretation of the results obtained, .
  5. Practicum that demonstrates the ability to perform accurate pipetting, metrology, interpret  experimental results and the ability to follow a protocol. 
  6. Instuctor assessment of student's class participation in discussions and group presentations regarding the use and application of techniques used or experimental results obtained. 

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Student Learning Outcomes

Outcome Text
Students will be able to explain cell structure, organization and function and how these basic cell components can be manipulated for industrial applications.

Required Texts

Specify Formatting Style
APA
Textbook (Minimum of 1 Required)
Recommended Yes
Condition and/or
Author(s) Seidman, L.A.
Title Basic Laboratory Calculations for Biotechnology
Edition 2nd
City
Publisher Pearson
Year 2008
Rationale
Industry standard.Definitive Text.
ISBN # 978-013223810
Textbook Transfer Data
Manual
Recommended No
Supplemental No
Condition
and
Author(s) Seidman, L.A., Moore, C. J.
Title Basic Laboratory Methods for Biotechnology
Date 01/01/2008
Publisher Pearson
Periodical
Software
Other Learning Materials

Library

Resource Needs
Library electronic resources will support this class No
N/A No
I recommend we add No
Materials in the Library support this class No
Other No
Legacy Additional Resources

Attached Files

Attach any required or supporting documents here. Supported file types include Word, PDF, Excel, and other similar file types.

Attached File
Approval Letter

Codes/Dates

Dates
School Approval
10/20/2015
Curriculum Committee Approval
02/09/2016
Board of Trustees
04/25/2016
State Approval
05/11/2016
Technical Change Comment
Originator Mackenzie, Emalee
Origination Date
2015-01-25
Implement Date
2016-02-29
Instructional Services
Effective Implementation date of new/revised course Fall 2016
Classification and Codes
Division ILT - Life Sciences and Technologies
Department IHS - Health Sciences
Program BIOTCH - Biotechnology
Subject BIOT
Suggested Catalog ID
993168.00
The auto-generated Catalog ID can be copied from the "Suggested Catalog ID" field above and pasted here. It can then be edited as necessary by Administrative user(s) only.
Catalog Id 14620.00
CB03 TOP Code
043000 - Biotechnology and Biomedical Technology
CB00 Course Control Number
CCC000573161
CB04 Course Credit Status
D - Credit - Degree Applicable
CB05 Course Transfer Status
A - Transferable to both UC and CSU.
CB08 Course Basic Skill Status (PBS Status)
N - Not Basic Skills
CB09 SAM Code
D - Possibly Occupational
CB10 Course COOP Work Exp-ED
N - Is not part of a cooperative work experience education program.
CB11 California Classification Codes
Y - Credit Course
CB13 Special Class Status
N - Course is not a special class.
CB21 Course Prior to College
Y - Not applicable
CB22 Non Credit Course Category
Y - Not Applicable, Credit course
CB23 Funding Agency Category
Y - Not Applicable (funding not used to develop course)
CB24 Program Course Status
Program Applicable
CB25 Course General Education Status
CB26 Course Support Course Status
CB27 Course Upper Division Status
CIP Code
Soc Code
Course Accounting Method
C - Census
Material Code
Required Prereq
Yes
Prereq Info
Prior completion of or concurrent enrollment in BIOT 70
Conditions of Enrollment Catalog View
Prereq Code
Prereq Earned
Required Coreq
No
Coreq Key
Coreq Course(s)
Admin Use Only

Methods of Instruction

Method of Instruction
Methods of Instruction
LAB - Laboratory Instruction (No Lecture)

ASSIST

Queue for ASSIST No
Last Request From Queue
Last Direct Request 08/19/2016

ASSIST Preview

Prefix BIOT
Course Number
70L
Content
Lab Content
  1. Biotechnology overview
    1. Biotechnology careers
    2. Good manufacturing practices
    3. Scientific Method
    4. Distinguish between Quantitative and Qualitative observations
  2. Basic laboratory techniques and metrology
    1. Good Laboratory Practices
    2. Lab safety guidelines
    3. pH meter
    4. Record keeping
    5. Accuracy verses precision
    6. Pipetting skills
    7. Spectrophotometer
    8. Metric system
    9. Graphs
    10. Microscope use and maintenance
  3. Protein technique
    1. Protein assays
    2. Introduction to enzymes
    3. Protein electrophoresis
  4. DNA techniques
    1. Basic bacteriology
    2. DNA electrophoresis
    3. Bacterial transformation
    4. Isolation of plasmid DNA/restriction digest
  5. Antibody methods
    1. ELISA assay
    2. Home pregnancy test
Course Description
This laboratory course addresses basic skills and techniques common to the biotechnology industry. Topics include measurement of activity and quantity of proteins, growth and manipulation of bacteria, genetic engineering and antibody methods. This course is intended for students majoring in applied biotechnology and is the recommended course to accompany BIOT 70.
Is Honors
No
Lecture Hours
0.0000
Lab Hours
49.8000
Outline Approval Date
2016-02-09
Outline Effective Date
2016-02-29
Prerequisites
Prerequisite
Prior completion of or concurrent enrollment in BIOT 70
Corequisites
Advisories
Enrollment Limitations
Objectives
  • Demonstrate accurate pipetting skills
  • Distinguish between accuracy and precision.
  • Explain the importance of Good laboratory Practices and record keeping.
  • Prepare and analyze graphs.
  • Perform a bacterial transformation.
  • Demonstrate use of aseptic techniques in microbiological applications.
  • Measure the quantity of isolated protein and nucleic using various lab instruments.
  • List the basic steps of the Enzyme Linked ImmunoSorbent Assay (ELISA) protocol and describe its use in medical diagnosis of disease or conditions (e.g. AIDS and pregnancy)
  • Identify microscope parts.
  • Employ a lab protocol and explain deviations from the protocol.
  • Demonstrate proper use and care of the microscope.
Instruction Methods
Laboratory Instruction (No Lecture)
Evaluation Methods
Other Texts
Assignments
  • Reading

    Laboratory instructions from lab manual, Equipment instructions, Web articles, standard operating procedures.

  • Writing

    Responses to worksheets questions based on interpretations of the results. Daily recordings in a laboratory notebook explaining lab protocols and analyzing results obtained. Written standard operating procedure document of an assigned method.

  • Oral Assignments

    Data compilation, graph preparation and interpretation.

  • Other Assignments

    Present individual team's findings to class.